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phase contrast images  (Nikon)


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    Nikon phase contrast images
    Phase Contrast Images, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 10098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 10098 article reviews
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    A Schematic representation of co-cultures. C2C12 and MLg cells were submitted to similar culture conditions for 6 days. At day 0 (D0), when C2C12 myotubes and MLg cells were fully confluent, EO771 cancer cells were seeded onto the cells. B <t>Representative</t> <t>phase-contrast</t> <t>microscopy</t> images of C2C12 and MLg stained with May-Grünwald and Giemsa at D0. Scale bar = 200 µm. C Number of EO771 cells per mm 2 attached onto C2C12 myotubes (C2C12 + EO771) and MLg cells (MLg + EO771) 3 h post-seeding at D0. D Outgrowth of EO771 cells (100 cells per well in 96-well plate) co-cultured with C2C12 myotubes (C2C12 + EO771) or MLg cells (MLg + EO771) for 2 days. EO771 area represents the percentage of fluorescent cells normalized to D0 ( n = 21–24 from three independent experiments). Representative fluorescence microscopy images of EO771 cell outgrowth on C2C12 or MLg at day 2 (D2). Scale bar = 200 µm. E Outgrowth of EO771 cells in co-cultures with C2C12 myotubes or MLg cells when seeded at 500, 1000, 4000, and 8000 cells per well in a 96-well plate at day 0. Green fluorescence of EO771 was measured over 2 days without normalization ( n = 24 from two independent experiments). F Representative fluorescence microscopy images of EO771 cell outgrowth (4000 cells per well in 96-well plate) on C2C12 or MLg. Scale bar = 200 µm. Data are mean ± SEM. Normality was tested with the Shapiro–Wilk test. Comparison between groups was done using the Mann–Whitney test (comparing two groups with one variable) or two-way ANOVA adjusted for multiple testing with the Šidák method (comparing two groups with two variables). ****P < 0.0001.
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    A Schematic representation of co-cultures. C2C12 and MLg cells were submitted to similar culture conditions for 6 days. At day 0 (D0), when C2C12 myotubes and MLg cells were fully confluent, EO771 cancer cells were seeded onto the cells. B <t>Representative</t> <t>phase-contrast</t> <t>microscopy</t> images of C2C12 and MLg stained with May-Grünwald and Giemsa at D0. Scale bar = 200 µm. C Number of EO771 cells per mm 2 attached onto C2C12 myotubes (C2C12 + EO771) and MLg cells (MLg + EO771) 3 h post-seeding at D0. D Outgrowth of EO771 cells (100 cells per well in 96-well plate) co-cultured with C2C12 myotubes (C2C12 + EO771) or MLg cells (MLg + EO771) for 2 days. EO771 area represents the percentage of fluorescent cells normalized to D0 ( n = 21–24 from three independent experiments). Representative fluorescence microscopy images of EO771 cell outgrowth on C2C12 or MLg at day 2 (D2). Scale bar = 200 µm. E Outgrowth of EO771 cells in co-cultures with C2C12 myotubes or MLg cells when seeded at 500, 1000, 4000, and 8000 cells per well in a 96-well plate at day 0. Green fluorescence of EO771 was measured over 2 days without normalization ( n = 24 from two independent experiments). F Representative fluorescence microscopy images of EO771 cell outgrowth (4000 cells per well in 96-well plate) on C2C12 or MLg. Scale bar = 200 µm. Data are mean ± SEM. Normality was tested with the Shapiro–Wilk test. Comparison between groups was done using the Mann–Whitney test (comparing two groups with one variable) or two-way ANOVA adjusted for multiple testing with the Šidák method (comparing two groups with two variables). ****P < 0.0001.
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    A Schematic representation of co-cultures. C2C12 and MLg cells were submitted to similar culture conditions for 6 days. At day 0 (D0), when C2C12 myotubes and MLg cells were fully confluent, EO771 cancer cells were seeded onto the cells. B <t>Representative</t> <t>phase-contrast</t> <t>microscopy</t> images of C2C12 and MLg stained with May-Grünwald and Giemsa at D0. Scale bar = 200 µm. C Number of EO771 cells per mm 2 attached onto C2C12 myotubes (C2C12 + EO771) and MLg cells (MLg + EO771) 3 h post-seeding at D0. D Outgrowth of EO771 cells (100 cells per well in 96-well plate) co-cultured with C2C12 myotubes (C2C12 + EO771) or MLg cells (MLg + EO771) for 2 days. EO771 area represents the percentage of fluorescent cells normalized to D0 ( n = 21–24 from three independent experiments). Representative fluorescence microscopy images of EO771 cell outgrowth on C2C12 or MLg at day 2 (D2). Scale bar = 200 µm. E Outgrowth of EO771 cells in co-cultures with C2C12 myotubes or MLg cells when seeded at 500, 1000, 4000, and 8000 cells per well in a 96-well plate at day 0. Green fluorescence of EO771 was measured over 2 days without normalization ( n = 24 from two independent experiments). F Representative fluorescence microscopy images of EO771 cell outgrowth (4000 cells per well in 96-well plate) on C2C12 or MLg. Scale bar = 200 µm. Data are mean ± SEM. Normality was tested with the Shapiro–Wilk test. Comparison between groups was done using the Mann–Whitney test (comparing two groups with one variable) or two-way ANOVA adjusted for multiple testing with the Šidák method (comparing two groups with two variables). ****P < 0.0001.
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    A Schematic representation of co-cultures. C2C12 and MLg cells were submitted to similar culture conditions for 6 days. At day 0 (D0), when C2C12 myotubes and MLg cells were fully confluent, EO771 cancer cells were seeded onto the cells. B <t>Representative</t> <t>phase-contrast</t> <t>microscopy</t> images of C2C12 and MLg stained with May-Grünwald and Giemsa at D0. Scale bar = 200 µm. C Number of EO771 cells per mm 2 attached onto C2C12 myotubes (C2C12 + EO771) and MLg cells (MLg + EO771) 3 h post-seeding at D0. D Outgrowth of EO771 cells (100 cells per well in 96-well plate) co-cultured with C2C12 myotubes (C2C12 + EO771) or MLg cells (MLg + EO771) for 2 days. EO771 area represents the percentage of fluorescent cells normalized to D0 ( n = 21–24 from three independent experiments). Representative fluorescence microscopy images of EO771 cell outgrowth on C2C12 or MLg at day 2 (D2). Scale bar = 200 µm. E Outgrowth of EO771 cells in co-cultures with C2C12 myotubes or MLg cells when seeded at 500, 1000, 4000, and 8000 cells per well in a 96-well plate at day 0. Green fluorescence of EO771 was measured over 2 days without normalization ( n = 24 from two independent experiments). F Representative fluorescence microscopy images of EO771 cell outgrowth (4000 cells per well in 96-well plate) on C2C12 or MLg. Scale bar = 200 µm. Data are mean ± SEM. Normality was tested with the Shapiro–Wilk test. Comparison between groups was done using the Mann–Whitney test (comparing two groups with one variable) or two-way ANOVA adjusted for multiple testing with the Šidák method (comparing two groups with two variables). ****P < 0.0001.
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    Image Search Results


    A Schematic representation of co-cultures. C2C12 and MLg cells were submitted to similar culture conditions for 6 days. At day 0 (D0), when C2C12 myotubes and MLg cells were fully confluent, EO771 cancer cells were seeded onto the cells. B Representative phase-contrast microscopy images of C2C12 and MLg stained with May-Grünwald and Giemsa at D0. Scale bar = 200 µm. C Number of EO771 cells per mm 2 attached onto C2C12 myotubes (C2C12 + EO771) and MLg cells (MLg + EO771) 3 h post-seeding at D0. D Outgrowth of EO771 cells (100 cells per well in 96-well plate) co-cultured with C2C12 myotubes (C2C12 + EO771) or MLg cells (MLg + EO771) for 2 days. EO771 area represents the percentage of fluorescent cells normalized to D0 ( n = 21–24 from three independent experiments). Representative fluorescence microscopy images of EO771 cell outgrowth on C2C12 or MLg at day 2 (D2). Scale bar = 200 µm. E Outgrowth of EO771 cells in co-cultures with C2C12 myotubes or MLg cells when seeded at 500, 1000, 4000, and 8000 cells per well in a 96-well plate at day 0. Green fluorescence of EO771 was measured over 2 days without normalization ( n = 24 from two independent experiments). F Representative fluorescence microscopy images of EO771 cell outgrowth (4000 cells per well in 96-well plate) on C2C12 or MLg. Scale bar = 200 µm. Data are mean ± SEM. Normality was tested with the Shapiro–Wilk test. Comparison between groups was done using the Mann–Whitney test (comparing two groups with one variable) or two-way ANOVA adjusted for multiple testing with the Šidák method (comparing two groups with two variables). ****P < 0.0001.

    Journal: Oncogenesis

    Article Title: Transcriptomic profiling of co-cultured cancer-host cells identifies hypoxia as a driver of the skeletal muscle cell’s anti-proliferative effect on cancer cells

    doi: 10.1038/s41389-026-00601-9

    Figure Lengend Snippet: A Schematic representation of co-cultures. C2C12 and MLg cells were submitted to similar culture conditions for 6 days. At day 0 (D0), when C2C12 myotubes and MLg cells were fully confluent, EO771 cancer cells were seeded onto the cells. B Representative phase-contrast microscopy images of C2C12 and MLg stained with May-Grünwald and Giemsa at D0. Scale bar = 200 µm. C Number of EO771 cells per mm 2 attached onto C2C12 myotubes (C2C12 + EO771) and MLg cells (MLg + EO771) 3 h post-seeding at D0. D Outgrowth of EO771 cells (100 cells per well in 96-well plate) co-cultured with C2C12 myotubes (C2C12 + EO771) or MLg cells (MLg + EO771) for 2 days. EO771 area represents the percentage of fluorescent cells normalized to D0 ( n = 21–24 from three independent experiments). Representative fluorescence microscopy images of EO771 cell outgrowth on C2C12 or MLg at day 2 (D2). Scale bar = 200 µm. E Outgrowth of EO771 cells in co-cultures with C2C12 myotubes or MLg cells when seeded at 500, 1000, 4000, and 8000 cells per well in a 96-well plate at day 0. Green fluorescence of EO771 was measured over 2 days without normalization ( n = 24 from two independent experiments). F Representative fluorescence microscopy images of EO771 cell outgrowth (4000 cells per well in 96-well plate) on C2C12 or MLg. Scale bar = 200 µm. Data are mean ± SEM. Normality was tested with the Shapiro–Wilk test. Comparison between groups was done using the Mann–Whitney test (comparing two groups with one variable) or two-way ANOVA adjusted for multiple testing with the Šidák method (comparing two groups with two variables). ****P < 0.0001.

    Article Snippet: Phase-contrast microscopy images were acquired using a CKX53 microscope with a UC90 color camera (Olympus, Tokyo, Japan) and a 10x objective, operated with Olympus cellSens software (version 2.2, Olympus).

    Techniques: Microscopy, Staining, Cell Culture, Fluorescence, Comparison, MANN-WHITNEY